The ntrA gene of Agrobacterium tumefaciens: identification, cloning, and phenotype of a site-directed mutant.
نویسندگان
چکیده
A 3.6-kb EcoRI fragment containing the ntrA gene of Agrobacterium tumefaciens was cloned by using the homologous ntrA gene of Rhizobium meliloti as a probe. Construction of an ntrA mutant of A. tumefaciens by site-directed insertional mutagenesis demonstrated the requirement of the ntrA gene for nitrate utilization and C4-dicarboxylate transport but not for vir gene expression or tumorigenesis.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 174 8 شماره
صفحات -
تاریخ انتشار 1992